KMID : 0043320090320040543
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Archives of Pharmacal Research 2009 Volume.32 No. 4 p.543 ~ p.547
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Difference in Growth Suppression and Apoptosis Induction of EGCG and EGC on Human Promyelocytic Leukemia HL-60 Cells
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Kim Jeong-Hee
Han Dong-Hoon
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Abstract
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Growth suppression and apoptosis inducing effect of (-)-epigallocatechin 3-gallate (EGCG) and (-)-epigallocatechin (EGC) were studied against human promyeolcytic leukemia, HL-60 cells. EGCG showed higher growth suppression against HL-60 cells than EGC. IC50 values for EGCG were 60.0 ¥ìM and EGC was 107.7 ¥ìM, respectively. Both EGCG and EGC induced apoptosis evidenced by nuclei fragmentation. Nuclear fragmentation was observed as a timedependent manner and the extent of nuclear fragmentation was slightly higher in EGCGtreated cells than EGC-treated cells. The expression level of Bcl-2 was decreased and caspase- 3 was activated by EGCG or EGC treatment. The extent in decrease of Bcl-2 and activation caspase-3 were more extensively occurred in EGCG-treated cells than in EGC-treated cells. These data corresponded to the growth suppression data. EGC showed no cytotoxicity to a normal V79-4 cell line and EGCG showed slight cytotoxicity at higher concentrations.
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KEYWORD
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EGCG, EGC, Growth suppression, Apoptosis, Bcl-2, Caspase-3
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